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        [KO Validated] N-Cadherin Rabbit mAb
        
                    
        
                        時(shí)間：2020-10-29 09:57   瀏覽次數(shù)：1966次
                    
        
                    
        
                    
        
                                            
                                            
        
                                                品牌
                                                ABclonal
                                                產(chǎn)品類型
                                                抗體
                                                
                                            
        
                                            
        
                                                產(chǎn)品名稱
                                                [KO Validated] N-Cadherin Rabbit mAb
                                                產(chǎn)品規(guī)格
                                                50ul /100ul /200ul
                                            
        
                                            
        
                                               產(chǎn)品目錄號(hào)
                                                A19083
                                                
                                                 產(chǎn)品目錄價(jià)
                                                800/1200/2000
                                            
        
                                           
                                                    
                                        
        
                        
        背景
        This gene encodes a classical cadherin and member of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone. [provided by RefSeq, Nov 2015]
        免疫原信息
        應(yīng)用
        反應(yīng)物種Human, Mouse, Rat
        驗(yàn)證應(yīng)用WBIHCIF
        推薦稀釋比&實(shí)驗(yàn)步驟
        • WB   1:500 - 1:2000  [ 實(shí)驗(yàn)步驟 ]
        • IHC   1:50 - 1:200  [ 實(shí)驗(yàn)步驟 ]
        • IF   1:50 - 1:200  [ 實(shí)驗(yàn)步驟 ]
        理論分子量140kDa
        實(shí)際分子量140kDa
        存儲(chǔ)緩沖液Store at -20℃. Avoid freeze / thaw cycles.
        Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
        實(shí)驗(yàn)方法Western blotting    
        陽(yáng)性樣本HeLa,293T,NIH/3T3,C6
        細(xì)胞定位
        純化方式Affinity purification
        [KO Validated] N-Cadherin Rabbit mAb 檢測(cè)圖片
        ABclonal:Western blot - [KO Validated] N-Cadherin Rabbit mAb (A19083) }
        Western blot - [KO Validated] N-Cadherin Rabbit mAb (A19083)
        Western blot analysis of extracts from normal (control) and N-Cadherin knockout (KO) HeLa cells, using N-Cadherin antibody (A19083) at 1:1000 dilution.
        Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
        Lysates/proteins: 25ug per lane.
        Blocking buffer: 3% nonfat dry milk in TBST.
        Detection: ECL Basic Kit (RM00020).
        Exposure time: 1min.
        ABclonal:Western blot - [KO Validated] N-Cadherin Rabbit mAb (A19083) }
        Western blot - [KO Validated] N-Cadherin Rabbit mAb (A19083)
        Western blot analysis of extracts of various cell lines, using N-Cadherin antibody (A19083) at 1:1000 dilution.
        Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
        Lysates/proteins: 25ug per lane.
        Blocking buffer: 3% nonfat dry milk in TBST.
        Detection: ECL Basic Kit (RM00020).
        Exposure time: 1min.
        ABclonal:Immunohistochemistry - [KO Validated] N-Cadherin Rabbit mAb (A19083) }
        Immunohistochemistry - [KO Validated] N-Cadherin Rabbit mAb (A19083)
        Immunohistochemistry of paraffin-embedded mouse heart using N-Cadherin Rabbit mAb (A19083) at dilution of 1:100 (40x lens).
        ABclonal:Immunohistochemistry - [KO Validated] N-Cadherin Rabbit mAb (A19083) }
        Immunohistochemistry - [KO Validated] N-Cadherin Rabbit mAb (A19083)
        Immunohistochemistry of paraffin-embedded rat heart using N-Cadherin Rabbit mAb (A19083) at dilution of 1:100 (40x lens).
        ABclonal:Immunofluorescence - [KO Validated] N-Cadherin Rabbit mAb (A19083) }
        Immunofluorescence - [KO Validated] N-Cadherin Rabbit mAb (A19083)
        Immunofluorescence analysis of mouse heart using N-Cadherin Rabbit mAb (A19083) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.